Key features and details | |
Cat. No. | MABL-3134 |
Name | Anti-TREM-1 mAbs |
Clone No. | AFD- 14F128 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, FC |
Species Reactivity | Human, Rhesus Monkey, Cynomolgus Monkey |
Basic Information | |
Specificity | This antibody binds human, cynomolgus monkey and rhesus monkey TREM-1. TREM1 is a receptor that is expressed on monocytes, macrophages, neutrophils and dendritic cells. It plays important roles in innate and adaptive immunity by amplifying inflammatory responses. When activated, TREM-1 associates with the ITAM-containing signaling adaptor protein, DAP12. Downstream signalling may include activation of the NFAT transcription factor, causing an upregulation of pro-inflammatory cytokine production. |
Alternative Name | TREM1; CD354; Triggering receptor expressed on myeloid cells 1; Triggering receptor expressed on monocytes 1 |
UniProt | Q9NP99 |
Immunogen | The original antibody was generated by immunizing both wild type BALB/c mice and TREM-1 knock-out (KO) mice (C57BL/6 background) with either human TREM-1, cells expressing hTREM-1 (BWZ.36 cells), or a combination of both. |
Application Notes | The original IgG1 antibody binds human TREM-1 with an affinity of 2.4 nM as tested by suface plasmon resonance. The screening of original antibody for binding human TREM-1 was done using ELISA and flow cytometry. This antibody is capable of binding both human, cynomolgus monkey and rhesus monkey PBMCs. This antibody was able to reduce activation of BWZ/hTREM-1 reporter cell. This antibody was also able to block the ligand-induced TREM-1 activation in such BWZ/hTREM-1 reporter cell assay (US9000127). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
