Key features and details | |
Cat. No. | MABL-3205 |
Name | Anti-VEGFR2 mAbs |
Clone No. | AFD- V2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the VEGFR2 protein. It shows no binding to individual domains 2-3, 1-3 and 1-4. VEGFR2 is a tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. It plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. |
Alternative Name | Vascular endothelial growth factor receptor 2; KDR; VEGFR-2; Fetal liver kinase 1; FLK-1; Kinase ｉｎｓｅｒｔ domain receptor; Protein-tyrosine kinase receptor flk-1; CD309 |
UniProt | P35968 |
Immunogen | The original human version of this antibody was created by screening a human phage display library with VEGFR2-Fc. |
Application Notes | The human version of this antibody was tested by ELISA with different domains of VEGFR2 (WO2017004254). Furthermore, binding of the human version of this antibody to human umbilical vein endothelial cells was demostrated using flow cytometry (WO2017004254). After that, HUVEC tube formation assays were preformed using the human version of this antibody. This antibody did not show significant inhibition of tube formation (WO2017004254). Further, a matrigel plug assay was preformed using the human version of this antibody to asses its ability to inhibit angiogenesis in vivo (WO2017004254). |
Antibody First Published | Wei-dong et al. Anti-vascular endothelial growth factor receptor 2 (vegfr2) antibodies WO2017004254 |
Note on publication | Describes the generation and characterization of this antibdy. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
