Key features and details | |
Cat. No. | MABL-3232 |
Name | Anti-VWFpp mAbs |
Clone No. | AFD- JA9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for VWF propeptide (VWFpp) and not to the yeast used for the expression of the propeptides. |
Alternative Name | von Willebrand factor propeptide |
UniProt | |
Immunogen | The original antibody was generated by selecting antibody fragments using phage display technology, where two phage display libraries were amplified and used to screen for binding to von Willebrand factor propeptide (VWFpp) displayed on yeast cells. |
Application Notes | This antibody's original format (scFv) was used in a sandwich ELISA to determine the VWF propeptide levels in plasma and showed a higher binding affinity for VWFpp in plasma at especially lower plasma concentrations (Meiring et al., 2018; PMID17:14 29992156). |
Antibody First Published | Meiring et al. The Use of Phage Display and Yeast Based Expression System for the Development of a Von Willebrand Factor Propeptide Assay: Development of a Von Willebrand Factor Propeptide Assay Biomed Res Int. 2018 May 24;2018:6232091. doi: 10.1155/2018/6232091 PMID:29992156 |
Note on publication | The original publication describes the development of a cost-effective assay for the diagnosis of von Willebrand disease, focusing on the detection of von Willebrand factor propeptide using phage display and yeast-based expression systems. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
