Anti-Vα TCR mAbs

Cat. No.

MABL-3234

Name

Anti-Vα TCR mAbs

Clone No.

AFD-34

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA, FC

Species Reactivity

Human

Basic Information

Specificity

This antibody binds to the T-cell receptor   alpha chains.

Alternative Name

TCR variable alpha chain; T-cell receptor   variable alpha chain

UniProt

P01848

Immunogen

Purified recombinant soluble TCR from the   T-cell hybridoma 85.33, corresponding to V alpha 11

Application Notes

The antibody binds specifically to the recombinant   and native variable domain of the α chain which comprises the T-cell receptor   (TCR). The TCR is a disulfide-linked heterodimeric protein expressed on the   surface of T-cells in complex with CD3 and ζ-chain molecules. The receptor  functions in the recognition of MHC molecule and the subsequent activation of  T-cells. The monoclonal antibody 34 recognises Vα regions of TCRs expressed in the 85.33 arthritogenic T-cell hybridoma and can be used to analyse TCR V   gene expression and for immunotherapy in T cell mediated autoimmunity.

Antibody First   Published

Popov S, Hubbard JG, Ward ES. A novel and   efficient route for the isolation of antibodies that recognise T cell   receptor V alpha(s). Mol Immunol. 1996 Apr;33(6):493-502. PMID:8700165

Note on publication

Describes the isolation of scFv fragments that recognise both the recombinant and native Vα chain expressed on the   surface of T-cells, and their characterization.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.