Key features and details | |
Cat. No. | MABL-3242 |
Name | Anti-WSSV mAbs |
Clone No. | AFD- A1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | White spot syndrome virus |
Basic Information | |
Specificity | The antibody is specific for WSSV. The antibody binds to native WSSV, and not to the denatured WSSV. |
Alternative Name | White spot syndrome virus |
UniProt | |
Immunogen | The original antibody was generated by immunizing mice with WSSV followed by antibody library construction and phage display-based selection. |
Application Notes | The binding specificity of the scFv antibody was determined by ELISA (Dai et al., 2003; PMID: 12969566 and Yuan et al., 2006; PMID: 17140131). The antibody had an affinity constant (Kaff) of 2.02 x 10^9 M-1. Dot blot assays showed that the antibody could detect WSSV directly from shrimp hemolymph after 24-h feeding infection by WSSV (Dai et al., 2003; PMID: 12969566). |
Antibody First Published | Dai et al. Construction and characterization of a novel recombinant single- chain variable fragment antibody against White Spot Syndrome Virus from shrimp J Immunol Methods. 2003 Aug;279(1-2):267-75. PMID:12969566 |
Note on publication | The original paper describes the generation and characterization of the antibody by phage display method. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
