Key features and details | |
Cat. No. | MABL-3246 |
Name | Anti-XCR1 mAbs |
Clone No. | AFD- MARX10 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vivo, FC, IHC |
Species Reactivity | Rat, Mouse |
Basic Information | |
Specificity | This antibody binds mouse XCR1, which is a receptor for chemokines SCYC1 and SCYC2 and expressed on 80% of CD8+ the dendritic cells. It transduces a signal by increasing the intracellular calcium ions level. This antibody is also reported to cross-reacts with rat CXR1. |
Alternative Name | mXCR1; Ccxcr1;Chemokine XC receptor 1; Lymphotactin receptor; SCM1 receptor; XC chemokine receptor 1;MARX1O |
UniProt | Q9R0M1 |
Immunogen | The original antibody was generated by immunizing B6.XCR1-lacZ mice every 3 weeks (4 times in succession) with 30 × 10^6 CD11c-enriched cells from C57BL/6 WT mice in combination with heat- inactivated B. pertussis. |
Application Notes | This antibody was used to stain cryostat sections (12 μm) of spleens from C57BL/6 WT and B6.XCR1-lacZ mice. It was found that this antibody could stain 20% of splenic conventional dendritic cells in C57BL/6 WT mice but did not give a signal in homozygous B6.XCR1-lacZ animals, which lack the XCR1 receptor. This antibody was also used to subsets of dendritic cells that express XCR1 using flow cytometry (Bachem et al., 2012; PMID: 22826713). Digoxigenin (Dig) coupled MARX10 was injected into C57BL/6 mice for in vivo labeling of XCR1+ dendritic cells. Recombinant ovalbumin (OVA) coupled MARX10 (MARX10-OVA) was used to measure antibody levels in C57BL/6 and homozygous B6.XCR1-lacZ mice serum following injection and for an in vivo cytotoxicity assay; MARX10-OVA was found to be highly effective in inducing CD8+ T cell cytotoxicity in vivo (Hartung et al., 2015; PMID: 25520399). |
Antibody First Published | Bachem et al. Expression of XCR1 Characterizes the Batf3-Dependent Lineage of Dendritic Cells Capable of Antigen Cross-Presentation. Front Immunol. 2012 Jul 18;3:214. PMID:22826713 |
Note on publication | XCR1 expression determines Batf3-dependent lineage of dendritic cells with a special capacity to cross-present antigen. The paper studies the expression of XCR1 on CD8+ dendritic cells using antibody MARX10 and its role in antigen presentation. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
