Key features and details | |
Cat. No. | MABL-274 |
Name | Anti- mouse Bim mAbs |
Clone No. | AFD- ham151-149 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, FC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | The antibody reacts with mouse Bim. Mouse Bim is a 19-24 kDa pro-apoptotic protein (Bcl-2 family) which regulates immunological responses. |
Alternative Name | Bcl-2-like protein 11; Bcl2-L-11; Bcl2-interacting mediator of cell death |
UniProt | O54918 |
Immunogen | The original antibody was generated from a hamster immunized with a complex of mouse Bim and Bcl-XL produced in insect cells. |
Application Notes | The antibody reacted in Western blots and by immunofluorescence with permeabilized B and T cells from Bim +/+ mice (Oliver et al., 2004; PMID: 15520248). T cells were analyzed for levels of Bcl-2 and Bim protein by staining of permeabilized cells with the antibody (Jorgensen et al., 2007; PMID: 17785775). The antibody was used for flow cytometry analysis (Kashiwakura et al., 2013; PMID: 23278598). |
Antibody First Published | Oliver et al. Loss of Bim Allows Precursor B Cell Survival But Not Precursor B Cell Differentiation in the Absence of Interleukin 7. J Exp Med. 2004 Nov 1;200(9)1179-87. PMID15520248 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
