Anti- α-gal mAbs

Cat. No.

MABL-276

Name

Anti- α-gal mAbs

Clone No.

AFD- B4H2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

lysis, ELISA

Species Reactivity

Cow, Rabbit, Mouse

Basic Information

Specificity

This antibody is specific for α-gal epitopes (Galα1–3Galβ1–4GlcNAc-R) on glycoprotein and glycolipid molecules.   Application Notes

Alternative Name

alpha gal; Galα1–3Galβ1–4GlcNAc-R; gal

UniProt

Immunogen

This antibody was raised by immunising   α1,3galactosyltransferase knock-out mice, which lack α-gal epitopes. Mice   were immunised with rabbit red cell membranes, on which α-gal is the major   carbohydrate epitope.

Application Notes

This antibody binds to glycoprotein   and glycolipid molecules expressing α-gal epitopes, including α-gal-BSA, in   ELISA analysis (Chen et al, 2005). It does not cross-react with any   glycoproteins lacking this epitope. This antibody binds to the α-gal epitopes   on the hybridoma cells producing the antibody, so can mediate complement   mediated autolysis in the presence of rabbit serum (Chen et al, 2000).

Antibody First Published

Chen et al. Genes coding evolutionary novel   anti-carbohydrate antibodies

Note on publication

Describes the original generation of this   antibody, and its characterisation in ELISA analysis.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.