Key features and details | |
Cat. No. | MABL-305 |
Name | Anti-25-hydroxyvitamin D mAbs |
Clone No. | AFD- AbAb2-VD |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | colloidal gold immunochromatography, ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody binds 25-hydroxyvitamin D. Vitamin D (calciferol) is an essential fat-soluble vitamin responsible for absorption and regulation of calcium and phosphorus, maintaining bone health, regulating immune function, preventing cardiovascular disease, and regulating fat and energy metabolism. |
Alternative Name | 25-OH-VD; 25(OH)D; vitamin D; calciferol |
UniProt | |
Immunogen | The original antibody was generated by subcutaneously immunizing New Zealand rabbits with a conjugate of 25-OH-VD and BSA emulsified with an equal amount of Freund's complete adjuvant. |
Application Notes | This antibody binds 25-hydroxyvitamin D in an ELISA with an EC50 of 4.72 ng/mL and the IgG version of the antibody as a binding affinity in the sub-picomolar range. This antibody in combination with AbAb1-VD can be used in the development of double-antibody sandwich colloidal gold immunochromatography test strips, where AbAb1-VD is used to prepare Au-antibody complex and AbAb2-VD is used for T line coating. |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
