Anti-3–5 ring polycyclic aromatic hydrocarbons (PAHs) mAbs

Cat. No.

MABL-307

Name

Anti-3–5 ring polycyclic aromatic hydrocarbons (PAHs) mAbs

Clone No.

From

Isotype

Engineer antibody

Application

ELISA

Species Reactivity

Species independent

Basic Information

Specificity

The antibody shows general selectivity and   high sensitivity to a variety of 3–5 ring PAH, but it shows a low affinity   for 2-ring PAHs. Polycyclic aromatic hydrocarbons (PAHs), a group of organic compounds composed of two or more blended aromatic rings, are an important   class of environmental pollutants

Alternative Name

unsubstituted PAH; 2-methylphenanthrene;9,10-dimethylanthracene; 7,12 dimethybenz(a)anthracene; 4-methyl dibenzothiophene; Benzo(a)pyrene; Chrysene; Pyrene; Phenanthrene; Anthracene

UniProt

Immunogen

The original antibody was generated by immunizing   Balb/c mice were with 1-pyrene butyric acid-KLH.

Application Notes

The specificity of the original format of   the antibody was confirmed by ELISA analysis. The antibody could inhibit   unsubstituted and methylated PAHs by indirect competitive ELISA. IC50 values for unsubstituted PAHs ranged from 1.68 μg/L to 9.44 μg/L, while IC50 values against methylated PAHs (2-methylphenanthrene, 9,10-dimethylanthracene, 7,12   dimethybenz(a)anthracene, 4-methyl dibenzothiophene) were ranging from 1.92   μg/L to 31 μg/L. The antibody was employed in the KinExA Inline Biosensor   system for quantifying 3–5 ring PAHs in aqueous environmental samples   (detection limit 0.2 μg/L) (Li et al., 2016; PMID: 26925369).

Antibody First Published

Li et al. A highly sensitive monoclonal antibody based biosensor for quantifying 3–5 ring polycyclic aromatic hydrocarbons (PAHs) in aqueous environmental samples. Sens Biosensing Res. 2016 Mar 1;7:115-120. PMID:26925369

Note on publication

The original paper describes the generation   and characterization of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.