Anti-ADAMts13 mAbs

Cat. No.

MABL-354

Name

Anti-ADAMts13 mAbs

Clone No.

AFD- 1-420 scFv

From

Recombinant Antibody

Isotype

Engineer antibody

Application

inhibition

Species Reactivity

Human, Mouse

Basic Information

Specificity

Binds to the ADAMTS13 spacer domain. 1-420 binds to the same ADAMTS13 spacer domain loop segments that are required for binding of ADAMTS13 to VWF. ADAMTS13 is a zinc-containing metalloprotease which is secreted in blood where it is able to cleave VWF, a protein involved in blood clotting, to decrease the protein's activity.

Alternative Name

vWF-CP; ADAM-TS13; ADAMTS-13; C9orf8; VWFCP;  ADAM metallopeptidase with thrombospondin type 1 motif 13

UniProt

A2ALB3

Immunogen

1-420 scFv was generated from a phage   display library derived from human TTP patients expressing autoantibodies   against ADAMTS13.

Application Notes

 Mice transfected with the scFv show ADAMTS13   inhibition and accumulation of unprocessed UL-VWF (ultralarge VWF) multimers.   1-420 mediates inhibition by binding to the same spacer domain loop domain   segments required for ADAMTS13 binding to VWF. Please note that this antibody   is subject to a pending patent application by the The Trustees Of The   University Of Pennsylvania (WO2016164468 A2).

Antibody First Published

Ostertag et al. ADAMTS13 autoantibodies   cloned from patients with acquired thrombotic thrombocytopenic purpura: 2.   Pathogenicity in an animal model Transfusion. 2016 Jul;56(7): 1775-85   PMID:27040023

Note on publication

"Describes the use of human anti-ADAMTS13 autoantibodies that function in mice to develop thrombotic thrombocytopenic purpura (TTP) disease model."

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.