Key features and details | |
Cat. No. | MABL-356 |
Name | Anti-Adeno associated virus mAbs |
Clone No. | AFD- A20 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, neutralizing, ELISA, IF |
Species Reactivity | Adeno-associated virus 2 |
Basic Information | |
Specificity | The antibody recognizes a conformational epitope on assembled capsid proteins of AAV-2 but it fails to detect nonassembled or denatured capsid proteins. |
Alternative Name | AAV-2 capsid proteins; Capsid protein VP1 |
UniProt | P03135 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with a mixture of recombinant AAV capsid proteins (VP1, VP2, and VP3). |
Application Notes | The clone was used to investigate the AAV assembly process on the cellular level. The clone was used to immunoprecipitate capsid proteins from extracts of [35S]methionine-labeled HeLa cells infected with AAV-2 and adenovirus type 2. Immunofluorescence was preformed on AAV-2/adenovirus type 2-coinfected HeLa cells using this antibody (Wistuba et al, 1997; pmid:8995658). Epitope sequences on the capsid surface were identified by enzyme-linked immunoabsorbent assay using AAV-2 mutants and AAV serotypes, peptide scan, and peptide competition experiments. The clone neutralizes infection following receptor attachment by binding an epitope formed during AAV-2 capsid assembly. The antibody bound empty and full AAV-2 particles in an ELISA. In a neutralization assay, incubation of rAAV-2-GFP with A20, on HeLa cells prevented transgene expression. The clone reacted also with AAV-3 as shown by ELISA (Wobus et al, 2000; pmid:10982375). |
Antibody First Published | Wistuba et al. Subcellular compartmentalization of adeno-associated virus type 2 assembly. J Virol. 1997 Feb;71(2):1341-52. PMID:8995658 |
Note on publication | The article describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
