Key features and details | |
Cat. No. | MABL-374 |
Name | Anti-alpha-5 TMD mAbs |
Clone No. | AFD- Nb25 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallisation |
Species Reactivity | Human |
Basic Information | |
Specificity | Binds to the transmembrane domain of the alpha-5 GABAA receptor. To see the exact epitope see the crystal structure (Miller et al, 2017). Nb25 straddles the neurotransmitter-binding site between adjacent subunits. |
Alternative Name | GABRA5; Gamma-aminobutyric acid receptor subunit alpha-5; GABA(A) receptor subunit alpha-5 |
UniProt | P31644; P28472 |
Immunogen | A llama was immunized six times with recombinant 1D4-tagged human a1b3 GABAARpentamer. Nanobodies were selected by phage display, and the clones were then screened for binding to the receptor. From these clones Nb25 was selected. |
Application Notes | The antibody was used to facilitate the crystallisation of the a5 GABAA receptor transmembrane domain where it was shown to form a 1080A2 area interface (approx.). Nb25 was used to increase the soluability and aid packing in the crystal. It has been shown that the binding of Nb25 does not affect the potentiation of the receptor. (Miller et al, 2017) |
Antibody First Published | Miller et al Structural basis for GABAA receptor potentiation by neurosteroids Nat Struct Mol Biol. 2017 Nov;24(11):986-992 PMID:28991263 |
Note on publication | Reports crystal structures of a chimeric GABAA receptor in both the apo and pregnanolone-bound states. Discusses the generation of this antibody, and its use in crystallisation of the GABAA receptor. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
