Key features and details | |
Cat. No. | MABL-394 |
Name | Anti-alpha-V beta 5 integrin mAbs |
Clone No. | AFD- EM09902 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA, FC, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the extracellular domain of human recombinant transmembranetruncated (DTM) αvβ5 integrin, a dimeric cell surface protein that mediates cell attachment. αvβ5 integrin is also involved in tumour growth and angiogenesis. |
Alternative Name | αvβ5; ITGAV: ITGB5; alpha V beta 5; alphaV beta5; alpha-V integrin; Vitronectin receptor subunit alpha; CD51; Integrin beta-5; ITGB5; P06756; P18084; aVb5 |
UniProt | |
Immunogen | This antibody was raised by immunising rabbits, isolating B cells from the immunised rabbits and fusing these with a rabbit plasmacytoma cell line (established by Spieker-Polet et al., 1995) to produce stable hybridomas |
Application Notes | This antibody has been used to specifically bind αvβ5 in Western blots and in flow cytometry (Goodman et al, 2012), revealing that cell lines HT29 and A549 strongly express αvβ5, with lower levels of expression by the M21 and M24 lines. This antibody also specifically stains αvβ5 in FFPE (formalin fixed paraffin while murine antibodies are typically unable to detect FFPE-embedded integrins. embedded)/TMA material (Goodman et al, 2012; Heß et al., 2014; Roth et l, 2013), |
Antibody First Published | Goodman et al. Matched rabbit monoclonal antibodies against αv-series integrins reveal a novel αvβ3-LIBS epitope, and permit routine staining of archival paraffin samples of human tumours Biol Open. 2012 Apr 15; 1(4): 329–340. PMID:23213423 |
Note on publication | Describes the generation and characterisation of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
