Anti-Arginase 1 mAbs

Cat. No.

MABL-438

Name

Anti-Arginase 1 mAbs

Clone No.

AFD- mAb1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Cryo-electron microscopy, inhibition

Species Reactivity

Human

Basic Information

Specificity

The antibody sterically blocks the arginase 1 active site near the exterior surface of the enzyme. Approximately 80% of all  complexes identified were composed of two arginase 1 trimers and three   antibodies, forming a 2 3 complex, while the remaining 20% of complexes were   composed of two arginase 1 trimers and two antibodies; however, the epitope and paratope interactions between each antibody and arginase 1 monomers are identical. Arginase is a manganese-containing enzyme that catalyzes the hydrolysis of L-arginine to L-ornithine and urea, and modulates   T-cell-mediated immune response.

Alternative Name

ARG1; Arginase-1; Liver-type arginase; Type I arginase

UniProt

P05089

Immunogen

The antibody is a human antibody identified   via yeast display technologies and isolated after panning against arginase 1.

Application Notes

The structure and epitope mapping of the   complex arginase antibody was determined by cryo-electron microscopy. The   antibody was assessed for its potency and extent of inhibition of human   arginase using an assay that detects the formation of thioornithine from   thioarginine. In this assay, as well as an LCMS assay, it was determined that   the antibody could fully inhibit the enzymatic activity of arginase. To assess the kinetic mechanism of inhibition of the antibody, increasing fixed concentrations of antibody were tested across a range of thioarginine   concentrations. The Ki for the antibody against human Arg1 was 3.3 ± 0.3 nM   (Palte et al., 2021; PMID: 34326456).

Antibody First Published

Palte et al. Cryo-EM structures of   inhibitory antibodies complexed with arginase 1 provide insight into   mechanism of action. Commun Biol. 2021 Jul 29;4(1):927. PMID:34326456

Note on publication

The paper describes the generation and   characterization of the antibody. The antibody was used as probes for Human   Arginase 1 conformational study.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.