Anti-Arsonate mAbs

Cat. No.

MABL-447

Name

Anti-Arsonate mAbs

Clone No.

AFD- 36-65

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallography, in vitro, in vivo, ELISA

Species Reactivity

Species independent

Basic Information

Specificity

The antibody is specific for arsonate.

Alternative Name

arsonate(1-); hydrogen arsonate; CHEBI: 64448; Q27133288; hydroxyarsinate

UniProt

Immunogen

The original antibody was generated by immunization A/J mice with the hapten p- azophenylarsonate.

Application Notes

The antibody is a relatively low-affinity   germline-encoded antibody with a true Ka of 2.5*10^5 M-1 (Rothstein et al.,   1983; PMID: 6843553). The specificity of the IgG1 format of the antibody was   confirmed by ELISA analysis. The antibody was employed in in vitro binding   assay, the antibody bound effectively to high density antigen beads with   retention of 57.8% while the binding to low density target was 17.6%. The   antibody behavior in vivo,using antigen-substituted beads s.c. implanted into   SCID mice was examined. Results showed the antibody bound effectively to the HD antigen target (Zuckier et al., 2000; PMID: 11156404). The crystal  structure of the Fab fragment was determined and the antibody affinity for the hapten was measured (Kd= 8.1 mM) (Sethi et al., 2006; PMID: 16618601).

Antibody First Published

Siekevitz et al. The genetic basis of   antibody production  the dominant anti-   arsonate idiotype response of the strain Aouse. Eur J Immunol. 1982   Dec;12(12):1023-32. PMID:6186498

Note on publication

The paper describes the generation and   characterization of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.