Anti-Asialoglycoprotein receptor mAbs

Cat. No.

MABL-451

Name

Anti-Asialoglycoprotein receptor mAbs

Clone No.

AFD- C2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

therapeutic, WB, IHC

Species Reactivity

Human

Basic Information

Specificity

C2 has been found to recognise denatured and   native forms of asialoglycoprotein receptor.

Alternative Name

ASGPR; ASGP-R; ASGR1; C-Type Lectin Domain   Family 4 Member H1; Hepatic Lectin H1; CLEC4H1; HL-1;

UniProt

P07306

Immunogen

A human non-immune phage antibody library   was screened with the recombinant carbohydrate recognition domain of rCRDH1   (the H1 subunit of human asialoglycoprotein receptor). Anti- rCRDH1 phage   clones were obtained after 4 rounds of screening with rCRDH1-coated   immunotubes and single positive colonies were further selected with the   expressed thioredoxin-His-S tag of the wild-type pET32c. Specific anti-rCRDH1   phage clones were transfected into E. coli HB2151 and induced for secreted   expression of scFv antibody.

Application Notes

C2 has been utilised in Western Blot and   immunohistochemical experiments in order to establish its ability to bind to   denatured ASGPR (Cao 2006). Since C2 is able to recognise denatured and   native forms of the ASGPR, the antibody could potentially be used to target   molecules for gene therapy of hepatocellular carcinoma or other liver   diseases (Cao 2006).

Antibody First Published

Cao et al. Characterization of a single-chain   variable fragment (scFv) antibody directed against the human   asialoglycoprotein receptor Biotechnol Appl Biochem. 2006 May;44(Pt 2):65-72.   PMID:16396634

Note on publication

Describes the generation of the C2 scFv and   its potential application for targeting molecules for liver diseases, in addition to the advantages of using scFvs to give greater targeting   specificity and better tumour penetration.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.