Key features and details | |
Cat. No. | MABL-454 |
Name | Anti-Astrovirus 2 mAbs |
Clone No. | AFD- PL-2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallography, IP, Neutralizing;SPR, ELISA |
Species Reactivity | Astrovirus 2 |
Basic Information | |
Specificity | The antibody binds to the surface of the HAstV-2 virion, which is formed by the virus capsid protein. The antibody binds to a conformation-dependent quaternary epitope on each side of the dimeric capsid spike. Strains of HAstV-2 (HAstV-2-Oxford, -RIVMa, -RIVMb, and -RIVMc) are resistant to neutralization by the antibody. |
Alternative Name | Human astrovirus 2; HAstV-2; Capsid polyprotein VP90; Capsid protein; HAstV-2 capsid spike protein |
UniProt | Q82446 |
Immunogen | The original antibody was isolated by immunizing BALB/c mice with H-Ast-2 virus. |
Application Notes | The original antibody (IgG2a) was used for immunofluorescence staining of H-Ast2- infected LLCMK2 cells. The antibody neutralizes HAstV serotype 2 (Sanchez-Fauquier et al., 1994; PMID: 7514320). The original antibody (IgG2a) and the scFv fragment bound to wheat germ extract containing recombinant HAstV-2 capsid protein, as confirmed by ELISA analysis. The crystal structure of the Fab fragment was determined. The scFv fragment was used to immunoprecipitate HAstV-2 capsid protein. This antibody could potentially be developed as a therapeutic and diagnostic agent (Bogdanoff et al., 2016; PMID: 27213181). The specificity of the antibody and scFv fragment for the HAstV-2 CP spike were confirmed by ELISA. The binding affinity of the Fab and scFv fragments towards the spike protein was found to be nearly identical (KD of 1.8 nM) using surface plasmon resonance. |
Antibody First Published | Sanchez-Fauquier et al. Characterization of a human astrovirus serotype 2 structural protein (VP26) that contains an epitope involved in virus neutralization Virology. 1994 Jun;201(2):312-20. doi: 10.1006/viro.1994.1296. PMID:7514320 |
Note on publication | The paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
