Key features and details | |
Cat. No. | MABL-477 |
Name | Anti-B7-H4 mAbs |
Clone No. | AFD- S2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, Block, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds a linear epitope comprising amino acids (VIQWLKEGVLGLVHEFK) of the human B7-H4. |
Alternative Name | B7H4; B7 homolog 4; B7h.5; O8E; Immune costimulatory protein B7-H4; B7S1; Protein B7S1; B7x; T-cell costimulatory molecule B7x; VTCN1; V-set domain-containing T-cell activation inhibitor 1 |
UniProt | Q7Z7D3 |
Immunogen | The original antibody was generated by immunizing female white leghorn chickens with 50 µg of recombinant B7-H4 protein (Phe29 to Ala258) through intramuscular injection. The spleens of the chickens were harvested 7 days after the final immunization to construct an scFv antibody library. The antibody was isolated by panning against B7-H4 protein. |
Application Notes | This antibody recognized intact wild type human B7-H4 and linear epitope comprising amino acids 'VIQWLKEGVLGLVHEFK' in an ELISA. This antibody was also capable or recognizing B7-H4 in a western blot assay. The binding of this antibody to B7-H4 protein can effectively neutralize the immunosuppression response induced by B7-H4 proteins, thus enabling T-cells to retain their activation status (PMID: 33716253). |
Antibody First Published | Lin et al. Generation of avian-derived anti-B7-H4 antibodies exerts a blockade effect on the immunosuppressive response. Exp Anim. 2021 Aug 6;70(3):333-343. PMID:33716253 |
Note on publication | Describes the generation of chicken antibodies against human B7-H4. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
