Key features and details | |
Cat. No. | MABL-488 |
Name | Anti-BAX mAbs |
Clone No. | AFD- 3C10 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallography, IP, WB, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for Bax. It recognizes a peptide encompassing residues 31–45 at the start of Bax a1–a2 loop (AAs RAGRM). |
Alternative Name | Apoptosis regulator BAX; Bcl-2-like protein 4 (Bcl2-L-4) |
UniProt | Q07812 |
Immunogen | |
Application Notes | The specificity of the antibody for Bax was determined by ELISA analysis. The antibody was able to bind to mitochondrial Bax and WT Bax by western blot analysis. The antibody immunoprecipitated Bax (Iyer et al., 2016; PMID： 27217060). The crystal structure of the Fab fragment in complex with BAX was determined. The antibody activates mitochondrial Bax, but blocks translocation of cytosolic Bax (Robin et al., 2018; PMID： 30122452). |
Antibody First Published | Robin et al. Identification of an activation site in Bak and mitochondrial Bax triggered by antibodies. Nat Commun. 2016 May 24;7 |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
