Key features and details | |
Cat. No. | MABL-495 |
Name | Anti-BCMA mAbs |
Clone No. | AFD- 17A5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, FC |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | This antibody binds human and mouse BCMA. It acts as a receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. BCMA expression is highest on terminally differentiated B cells. BCMA is involved in mediating the survival of plasma cells for maintaining long-term humoral immunity. It also activates NF- kappa-B and JNK. The expression of BCMA has been linked to a number of cancers, autoimmune disorders, and infectious diseases. |
Alternative Name | CD269; BCM; TNFRSF17; Tumor necrosis factor receptor superfamily member 17; B-cell maturation protein |
UniProt | Q02223 |
Immunogen | The original antibody was isolated from a generic Fab antibody library by panning against biotinylated ectodomain of BCMA. |
Application Notes | The selection of specific binders having affinity for BCMA was done using ELISA and their affinity was determined using SPR analysis. The original Fab antibody bound human and mouse BCMA with a monovalent binding affinity of 45 nM and 74 nM respectively. The binding of the IgG version of this antibody to membrane bound human BCMA was determined by performing flow cytometry on BCMA- expressing H929 cells and MKN45 cells. The assessment of the competition between A-APRIL and this antibody was performed on H929 cells by quantifying the binding of A-APRIL in presence of increasing concentrations of this antibody and measured by flow cytometry. This antibody was reported to not block or increase APRIL-dependent NF-κB activation and also does not block or increase BAFF-dependent NF-κB activation in vitro. |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
