Key features and details | |
Cat. No. | MABL-498 |
Name | Anti-BCMA mAbs |
Clone No. | AFD- CA8 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, FC |
Species Reactivity | Human, Cynomolgus Monkey |
Basic Information | |
Specificity | This antibody binds human and cynomolgus monkey BCMA and does not bind TACI or BAFF-R proteins. BCMA acts as a receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. BCMA expression is highest on terminally differentiated B cells. BCMA is involved in mediating the survival of plasma cells for maintaining long-term humoral immunity. It also activates NF-kappa-B and JNK. The expression of BCMA has been linked to a number of cancers, autoimmune disorders, and infectious diseases. |
Alternative Name | CD269; BCM; TNFRSF17; Tumor necrosis factor receptor superfamily member 17; B-cell maturation protein; S307118G03; 118G03 |
UniProt | Q02224 |
Immunogen | The original mouse parental antibody 118G03 was generated by immunizing SJL mice with recombinant human BCMA/TNFRSF17-Fc chimera (R&D 193-Fc) by using the RIMMS method (Rapid immunization multiple sites). A chimeric version of this antibody was generated and it is called CA8. |
Application Notes | The binding characterization of this antibody towards human and cynomolgus monkey recombinant BCMA was done using ELISA (US9273141). The binding of the antibody to membrane bound BCMA expressed on HEK293, H929, OPM-2, JJN-3 and U266 cells was done using flow cytometry (US9273141). This antibody neutralizes binding of both BCMA ligands BAFF and APRIL, and subsequently blocks the induction of NF-κB signaling cascades (PMID： 26370838). The EC50 values for chimeric CA8 were calculated to be 0.695 μg/mL and 0.773 μg/mL respectively (US9273141). This antibody neutralized both BAFF and APRIL induced NfkappaB cell signaling in H-929 cells. |
Antibody First Published | Yu-Tzu Tai et al. Targeting B-cell maturation antigen in multiple myeloma. Immunotherapy. 2015 Nov; 7(11)： 1187–1199. PMID：26370838 |
Note on publication | Discusses the novel immunotherapy treatment for multiple myelomas by targeting a cell surface protein like B-cell maturation antigen. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
