Anti-BCR-ABL mAbs

Cat. No.

MABL-500

Name

Anti-BCR-ABL mAbs

Clone No.

AFD- 7c6

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, WB, IF, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for the SSINEEITPRRQS   epitope of BCR (residues 686-696), and as such is able to recognise   full-length BCR and the p210 form of the BCR-ABL fusion protein. p210 BCR-ABL   is a cytogenetic marker of chronic myelogenous leukaemia, resulting from   gross chromosomal translocation resulting in the 'Philadelphia chromosome'.   The fusion protein is a constitutively active tyrosine kinase, causing   deregulated downstream signalling and increasing likelihood of oncogenic   transformation.

Alternative Name

BCR/ABL; Breakpoint cluster region   protein-tyrosine protein kinase ABL1 fusion protein

UniProt

Immunogen

Murine antibodies were produced by immunising BALB/c mice with residues 686-696 (SSINEEITPRRQS) of human BCR   fused to thyroglobulin. Hybridomas were generated by fusion of immunised   cells with the myeloma cell line P3X63Ag8 via established methods.

Application Notes

It has been shown by Western blot that 7c6   binds wildtype, full-length BCR and the p210 BCR-ABL fusion protein.

Antibody First Published

Dhut et al. Identification of two normal bcr   gene products in the cytoplasm. Oncogene. 1988 Nov;3(5)：561-6 PMID：3078961

Note on publication

Describes the production of 7c6 using a   synthetic BCR peptide (corresponding to residues 686-696) and the subsequent   use of the antibody in detecting normal BCR and various BCR-ABL fusion gene   products.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.