Anti-beta 3 integrin mAbs

Cat. No.

MABL-507

Name

Anti-beta 3 integrin mAbs

Clone No.

AFD- EM00212

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IHC;

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for the cytoplasmic domain (amino acids 742 to 788) of integrin β3.

Alternative Name

β3A; Platelet membrane glycoprotein IIIa; CD61; b3

UniProt

P05106

Immunogen

This antibody was raised by immunising   rabbits, isolating B cells from the immunised rabbits and fusing these with a   rabbit plasmacytoma cell line (established by Spieker-Polet et al., 1995) to   produce stable hybridomas

Application Notes

This antibody has been used to stain   β3-containing integrins in FFPE (formalin fixed paraffin embedded)/TMA   material, where murine antibodies function poorly (Goodman et al, 2012; Heß   et al., 2014).

Antibody First Published

Goodman et al. Matched rabbit monoclonal   antibodies against αv-series integrins reveal a novel αvβ3-LIBS epitope, and   permit routine staining of archival paraffin samples of human tumours Biol   Open. 2012 Apr 15; 1(4): 329–340. PMID:23213423

Note on publication

Describes the generation and characterisation of this antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.