Key features and details | |
Cat. No. | MABL-514 |
Name | Anti-Beta Lactamase mAbs |
Clone No. | AFD- cAbTEM13 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | inhibit, ELISA |
Species Reactivity | Escherichia coli |
Basic Information | |
Specificity | This antibody binds an enzyme called beta-lactamase produced by bacteria, which catalysis the breaking of the beta-lactam ring of beta-lactam antibiotics such as penicillin, cephalosporin, cephamycin and monobactam through hydrolysis. The breaking of the beta-lactam ring deactivates the antibacterial properties of the antibiotic molecule and provides antibiotic resistance in bacteria. |
Alternative Name | β-Lactamase; bla; Beta-lactamase TEM; IRT-4; Penicillinase; TEM-1 β- lactamase; cAb-TEM13 |
UniProt | P62593 |
Immunogen | The original antibody was generated by immunizing adult dromedary (Camelus dromedarius) with TEM-1 β-lactamase. |
Application Notes | The binding reactivity of this antibody to TEM-1 beta-lactamase was determined using ELISA. This antibody was capable of inhibiting beta-lactamase activity in vitro with an IC50 of 0.08 μM. The in vivo inhibition activity of this antibody to inhibit surface-exposed TEM-1 and prevent bacterial growth was assessed by preincubating cells expressing TEM-1 with increasing amounts of the antibody fragment. It was reported that when cells were preincubated with cAbTem13 before plating lost their ability to neutralize the antibiotic ampicillin (PMID: 11557473). |
Antibody First Published | Conrath et al. β-Lactamase inhibitors derived from single-domain antibody fragments elicited in the Camelidae. Antimicrob Agents Chemother. 2001 Oct; 45(10): 2807–2812.PMID:11557473 |
Note on publication | Describes the generation of B-lactamase inhibitory antibodies derived from camels. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
