Key features and details | |
Cat. No. | MABL-517 |
Name | Anti-Beta-actin mAbs |
Clone No. | AFD- Fab 19, actin |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the pointed end of actin filaments (Brawley et al, 2010; pmid: 21103060). Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed: 21103060). Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed: 29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. |
Alternative Name | Actin, cytoplasmic 1; Fab 19; Fab19; Fab 19, actin; Actin, cytoplasmic 1; ACTB; Beta-actin |
UniProt | P60709 |
Immunogen | The original Fab version of this antibody was obtained by recombinant antibody phage display. |
Application Notes | Immunofluorescence was done with this antibody on actin filaments. In this experiment the antibodies bound to the pointed end of actin (Brawley et al, 2010; pmid: 21103060). ELISA was also preformed with this antibody on actin filaments. This was to confirm the binding of the antibody to actin filaments (Brawley et al, 2010; pmid: 21103060). |
Antibody First Published | Brawley et al. Characterization of engineered actin binding proteins that control filament assembly and structure PLoS One. 2010 Nov 12;5(11):e13960. PMID:21103060 |
Note on publication | Describes the use of phage display mutagenesis to generate new class of binding protein that can be targeted to the pointed-end of actin. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
