Anti-Botulinum neurotoxin type A mAbs

Cat. No.

MABL-537

Name

Anti-Botulinum neurotoxin type A  mAbs

Clone No.

AFD- CR1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

neutralize, FC

Species Reactivity

Clostridium

Basic Information

Specificity

CR1 binds to both BoNT/A1 and BoNT/A2 with   high affinity (CR1 scfv: KD BoNT/A1 ~ 0.13 nM; KD BoNT/A2 ~ 0.4 nM. CR1 IgG:   KD BoNT/A1 ~ 2.5 pM; KD BoNT/A2 ~ 1.7 nM.). CR1 Fab fragment binds to the   BoNT/A1 binding domain, at the interface between the N-terminal lectin   subdomain (HCN) and the C- terminal trefoil subdomain (HCC) at a   discontinuous epitope consisting of β23-β24, β25-β26, β27-β28 and β35-β36   loops of the lectin subdomain and the C-terminus of the trefoil. Botulinum   neurotoxin (BoNT) is secreted by Clostridial bacteria, and causes botulism   which is characterized by flaccid paralysis and can be fatal without   hospitalization. BoNT acts by inhibitnig acetylcholine release through   binding with high affinity to SV2 on the presynaptic membrane and inducing   its internalization by receptor-mediated endocytosis.

Alternative Name

BoNT/A; BoNT; Botulinum neurotoxin; Bontoxilysin-A; Bontoxilysin A; BOTOX

UniProt

P10845

Immunogen

CR1 was produced by a yeast gene diversity   library used to increase the affinitiy of scFv AR2 for BoNT/A2, while   maintinaing high affinity binding to BoNT/A2. AR2 was generated after   multiple rounds of affinity maturation of the humanized C25, which itself was   created by CDR-grafting from the parental murine C25. Murine C25 was prepared   by constructing a scFv phage display library from mice immunized with   recombinant BoNT/A binding domain (Hc).

Application Notes

Antibody binding can be studied by FC and   the antibody also shows in vivo neutralizing activity against botulinum   neurotoxin - the neutralizing activity is increased when administered in combination with the human BoNT/A antibodies RAZ1 and 2G11.

Antibody First Published

Garcia-Rodriguez et al. Molecular evolution of antibody cross-reactivity for two subtypes of type A botulinum neurotoxin.  Nat Biotechnol. 2007 Jan;25(1):107-16.PMID17173035

Note on publication

Describes the process of increasing AR2   antibody affinity for BoNT/A2 while maintaining high affinity binding to   BoNT/A1. Thermodynamics of antiody binding to BoNT/A1 and BoNT/A2 were   studied and the crsytal structure of the antibody complexed with BoNT/A1 was   solved.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.