Anti-Brucella A antigen mAbs

Cat. No.

MABL-557

Name

Anti-Brucella A antigen mAbs

Clone No.

AFD- YsT9.1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

functional assay, SPR, ELISA

Species Reactivity

Brucella

Basic Information

Specificity

This antibody is specific for the brucella class of bacteria. Brucella is a genus of Gram-negative bacteria, named after David Bruce (1855–1931). They are small (0.5 to 0.7 by 0.6 to 1.5 µm), nonencapsulated, nonmotile, facultatively intracellular coccobacilli.

Alternative Name

YsT9-1

UniProt

Immunogen

This antibody was raised by immunizing mice   with B.melitensis.

Application Notes

To characterize the binding of this   antibody, an ELISA was preformed on a B. melitensis 16M   O-polysaccharide-biotin conjugate using the mouse version of this antibody   (Bundle et al, 1989; pmid:2474505). An anti-idiotypic antibody was raised by  panning against the mouse version of this antibody. Furthermore surface plasmon resonace was preformed using an anti-idiotipic antibody with the   mouse version of this antibody as antigen (Tanha et al, 2002; pmid:12009207).

Antibody First Published

Bundle et al. Definition of Brucella A and M epitopes by monoclonal typing reagents and synthetic oligosaccharides. Infect Immun. 1989 Sep; 57(9): 2829–2836. PMID:2474505

Note on publication

The paradigm that Brucella A and M epitopes   are simultaneously expressed on single cells and within one antigen molecule   was reinvestigated by using polysaccharide-specific murine monoclonal   antibodies.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.