Anti-C5a mAbs

Cat. No.

MABL-575

Name

Anti-C5a mAbs

Clone No.

AFD- Fab 31

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA

Species Reactivity

Human

Basic Information

Specificity

Fab 31 recognises both C5 and C5a.

Alternative Name

Complement Component 5a; Complement C5; C3   and PZP-Like Alpha-2- Macroglobulin Domain Containing Protein 4; Complement   Component 5; C5a Anaphylatoxin; Prepro-C5; CPAMD4; Anaphylatoxin C5a Analog;   ECLZB; C5D; C5b

UniProt

P01031

Immunogen

A panel of mAbs against the activated   complement component C5a was obtained from a filamentous phage M13-Fab   display library generated from mice immunized with human rC5a. Fabs isolated   from the library after iterative selection against rC5a bound to both rC5a   and purified C5. To isolate Fabs specific for neoepitopes expressed on C5a   but not on the native complement component C5, the library was rescreened in   a competitive manner. The phage Fab library was first incubated with   immobilized C5 to deplete C5 reactive Fabs. The C5 nonadherent phage were   then incubated with immobilized rC5a in the presence of soluble C5. Bound   phage were eluted and subjected to two additional cycles of subtraction with   immobilized C5 and selection with immobilized rC5a in the presence of soluble   C5. After three cycles of this competitive biopanning, Fabs reactive with   rC5a were isolated.

Application Notes

Antibody First Published

Ames et al. Isolation of Neutralizing   Anti-C5a Monoclonal Antibodies from a Filamentous Phage Monovalent Fab   Display Library Journal of Immunology, 1994, 152： 4572. PMID：8157971

Note on publication

Describes the generation of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.