Key features and details | |
Cat. No. | MABL-577 |
Name | Anti-C5aR mAbs |
Clone No. | AFD- S5/1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, Functional Studies, IP, WB, ELISA, FC |
Species Reactivity | Cow, Ferret, Rabbit, Human |
Basic Information | |
Specificity | S5/1 antibody recognizes the C5aR (CD88) protein, a 43 kDa receptor of C5a component of the complement cascade. |
Alternative Name | CD88; C5a anaphylatoxin chemotactic receptor; C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin chemotactic receptor; C5a-R; C5aR |
UniProt | P21730 |
Immunogen | This antibody was raised by immunizing mice with a synthetic peptide corresponding to C5a- R; amino acids： 1-31 (N terminal). |
Application Notes | S5/1 antibody inhibits C5a binding to its receptor C5aR (Oppermann et al., 1993). Furthermore, this antibody has been widely applied in the detection and analysis of C5aR via various methods. For instance, it was used in a flow cytometric study on peripheral blood lymphocytes where it was shown to bind to monocytes and granulocytes (Oppermann et al., 1993). It was also utilized in the Western blot experiments comparing C5aR expression in different groups of mice (Panayiotou et al., 2017). S5/1 was also successfull in labelling C5aR in the paraffin-embedded immunohistochemical samples of umbilical arteries (Fortin et al., 2002). |
Antibody First Published | Oppermann et al. Probing the human receptor for C5a anaphylatoxin with site-directed antibodies. Identification of a potential ligand binding site on the NH2-terminal domain. J Immunol. 1993 Oct 1;151(7)：3785-94. PMID：8376805 |
Note on publication | This article describes the generation and characterization of the S5/1 antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
