Anti-Ca 19-9 mAbs

Cat. No.

MABL-579

Name

Anti-Ca 19-9 mAbs

Clone No.

AFD- 121SLE

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ICC, ELISA, FC, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for CA19-9.

Alternative Name

121 SLE; sialyl Lewis a

UniProt

Immunogen

The original antibody was raised by   immunizing a mouse with ten precipitin lines obtained after immunodiffusion   using monoclonal antibody NS 19-9 and mucins isolated from an ovarian cyst of   a OLe (a+b-) patient.

Application Notes

The original version of this antibody was   used for immunohistochemistry on the normal gastrointestinal mucosa of   Le-positive individuals (Herrero-Zabaleta et al., 1987; PMID：2444292), and on   endometrial tissue specimens obtained in different phases of the menstrual   cycle (Hey and Aplin., 1996; PMID：8910004). This antibody was used for an ELISA on carbohydrate proteins (Manimala et al., 2007; PMID：17483136), and on MUC1 samples (Hey and Aplin., 1996; PMID：8910004). Furthermore   this antibody was used for immunocytochemistry on HT 29 tumour cells (Köhler   et al., 2009; PMID：20010946). The original version of this antibody was used for flow   cytometry on ovarian cancer cell line SW626 and SCLC cell line DMS79   (Ragupathi et al., 2009; PMID：19190907).

Antibody First Published

Herrero-Zabaleta et al. Monoclonal antibody against sialylated Lewis(a) antigen Bull Cancer. 1987;74(4):387-96. PMID:2444292

Note on publication

The original paper isolated hybridoma cells,   which secreted monoclonal antibody (MAb) 121 SLE.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.