Key features and details | |
Cat. No. | MABL-591 |
Name | Anti-calf thymus ATPaseA mAbs |
Clone No. | AFD- 6E12-C1-H2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA |
Species Reactivity | Cow |
Basic Information | |
Specificity | This antibody is specific for calf thymus ATPase A and recognizes the c-terminus of native bovine, human, and mouse homologs. Calf ATPaseA is a TP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. The antibody shows very little reaction to denatured protein. |
Alternative Name | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1; HepA-related protein; Sucrose nonfermenting protein 2-like 1 |
UniProt | Q9TTA5 |
Immunogen | The mouse version of this antibody was generated by immunizing mice with immunopurified calf thymus ATPaseA. |
Application Notes | The mouse version of this antibody was used for a western blot on calf DNA- dependent ATPase A isolated from calf thymus using immunoprecipitation (Mesner et al, 1993; pmid:8394114). To identify the mouse version of this antibody an ELISA on native ATPase A was preformed. Furthermore, a western blot was preformed on bacteriophage T4 gene 44/62 and 45 proteins. It showed very little reaction (Mesner et al, 1991; pmid:1720977). |
Antibody First Published | Mesner et al. DNA-dependent adenosinetriphosphatase A is the eukaryotic analogue of the bacteriophage T4 gene 44 protein: immunological identity of DNA replication-associated ATPases Biochemistry. 1991 Dec 10;30(49):11490-4. PMID:1720977 |
Note on publication | The construction of three stable murine hybridomas that secrete monoclonal antibodies which recognize calf thymus DNA-dependent adenosinetriphosphatase A is reported. All three of the antibodies react specifically with calf thymus ATPase A and the gene 44 protein from the bacteriophage T4 DNA-dependent ATPase. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
