Key features and details | |
Cat. No. | MABL-619 |
Name | Anti-Carbonic Anhydrase IX mAbs |
Clone No. | AFD- M75 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, crystallography, IB, ITC, WB, ELISA, FC, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the sequence PGEEDLP, which is repeated several times between the 61st and 96th aa residues of human carbonic anhydrase IX's PG domain. |
Alternative Name | CA9; CAIX; MN/CA IX; endogenous protein p54/58N |
UniProt | Q16790 |
Immunogen | The original antibody was generated by immunizing BALB/C mice with MaTu-infected HeLa cells. |
Application Notes | The original format of this antibody (mouse IgG2b) was characterized by and used in IB (WB), IF, and FC assays (Pastoreková et al., 1992; PMID: 1312272). This antibody's epitope has been determined by a competitive ELISA against the peptide APGEEDLPA. Furthermore, it was used for WB and was shown to blocks the capacity of immobilized MN/CA IX to attach cells (Závada et al., 2000; PMID: 10839295). The Fab version of this antibody was crystallized and analyzed. Additionally, an scFv version of this antibody was created, and its thermodynamic parameters against the peptide DLPGEEDLPGEE were evaluated via ITC. Its Kd was determined to be 0.42 μM (Král et al., 2008; PMID: 18041760). This antibody was used for WB in breast cell lines and for IHC in xenograft tumors grown from PC-3 cells in mice (Li et al.,2009; PMID: 19538935). |
Antibody First Published | Pastoreková et al. A novel-quasi-viral agent, MaTu, is a two-component system. Virology. 1992 Apr;187(2):620-6. PMID:1312272 |
Note on publication | The original publication introduces MaTu, a quasi-viral agent derived from a human mammary tumor, and details the generation and characterization of monoclonal antibodies against it. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
