Anti-Caspase-3 mAbs

Cat. No.

MABL-631

Name

Anti-Caspase-3 mAbs

Clone No.

AFD-VhhCasp32

From

Recombinant Antibody

Isotype

Engineer antibody

Application

in vitro, WB

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human   and sheep Caspase-3

Alternative Name

Casp2; CASP3; CPP-32; SCA-1; Apopain; Protein Yama; SREBP cleavage activity 1; Cysteine protease CPP32; 3.4.22.56

UniProt

P42574

Immunogen

The original antibody was raised by   panning against a llama phage display library.

Application Notes

The original antibody was used for a   western blot against recombinant Caspase 3. This antibody was expressed in   SHSY-5Y and the effect of the antibodies on apoptosis in presence of H2O2. It   showed that it indeed decreased apoptosis under oxidative stress (McGonigal   et al., 2009; PMID:18553063).

Antibody First Published

McGonigal et al. Isolation and   functional characterization of single domain antibody modulators of Caspase-3   and apoptosis Appl Biochem Biotechnol. 2009   May;157(2):226-36. PMID:18553063

Note on publication

In the original paper two   V(H)Hs specific to Caspase 3 (VhhCasp31 and VhhCasp32) were isolated from a   heavy chain antibody variable domain (V(H)H) phage display library and tested   for their apoptosis-modulating effects.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.