Key features and details | |
Cat. No. | MABL-642 |
Name | Anti-CCL18 mAbs |
Clone No. | AFD- AZN-CK18 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, ELISA, FC, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody specifically reacts with CCL18 in both direct and sandwich ELISA (Lindhout et al, 2001, pmid:11207283) (van der Voort et al., 2005, pmid: 15713699). The antibody does not cross-react with MIP1α (CCL3), MIP1β (CCL4), HCC-1 (CCL14), TARC (CCL17), MDC (CCL22), MIP3β (CCL19), MCP-1 (CCL2), RANTES (CCL5), or MCP-3 (CCL7) (Lindhout et al, 2001, pmid:11207283) (van der Voort et al., 2005). |
Alternative Name | DC-CKI; C-C motif chemokine 18; AMAC-1; MIP-4; CC chemokine PARC; Small-inducible cytokine A18; 3B8 |
UniProt | P55774 |
Immunogen | BALB/c mice were immunized with rhCCL18/DC-CK1 coupled to keyhole limpet hemocyanin weekly. After 5 weeks, splenocytes were isolated and used to obtain hybridomas. |
Application Notes | This antibody can be used both in direct and sandwich ELISA (van der Voort et al., 2005). The antibody was shown to work in immunohistochemistry (Lindhout et al, 2001).Immunopercipitation can be done using this antibody (van der Voort et al., 2005). The antibody is also reccomended for flow cytometry. |
Antibody First Published | van der Voort. et al Novel monoclonal antibodies detect elevated levels of the chemokine CCL18/DC‐CK1 in serum and body fluids in pathological conditions. J Leukoc Biol. 2005 May;77(5):739-47 PMID:15713699 |
Note on publication | Describes the generation of the antibody and the specificity testing of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
