Key features and details | |
Cat. No. | MABL-652 |
Name | Anti-CCR5 (phosphoserine 337) mAbs |
Clone No. | AFD- V14/2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, ELISA, FC, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | V14/2 antibody has been demonstrated to exclusively recognise CCR5 (CD195) phosphorylated at Ser 337. |
Alternative Name | CD195; C-C chemokine receptor type 5; C-C CKR-5; CC-CKR-5; CCR-5; CHEMR13; HIV-1 fusion coreceptor; p337; pS337; Ser-337; Ser337 |
UniProt | P51681 |
Immunogen | This antibody was raised by immunizing mice with a synthetic phospho peptide corresponding to the C-Terminal region of human CCR5 conjugated to BSA. |
Application Notes | The V14/2 antibody is recommended for the specific detection and analysis of CCR5 phosphorylated on serine 337 (Pollok-Kopp et al., 2003). It was used in the detection of pS337 CCR5 in lysates of RBL-2H3 cells via Western blot (Pollok-Kopp et al., 2003). With other anti-CCR5 antibodies which react with nonphosporylated CCR5 (Ab01577) or CCR5 phosphorylated on different serine residues (Ab01578), it can be used to study dynamic regulation of CCR5 and of phosphorylation mechanisms. |
Antibody First Published | Pollok-Koppet et al. Analysis of ligand-stimulated CC chemokine receptor 5 (CCR5) phosphorylation in intact cells using phosphosite-specific antibodies. J Biol Chem. 2003 Jan 24;278(4):2190-8. Epub 2002 Oct 27. PMID:12403770 |
Note on publication | This article describes the generation and characterization of the V14/2 antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
