Key features and details | |
Cat. No. | MABL-661 |
Name | Anti-CCRL2 mAbs |
Clone No. | AFD- BZ2E3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assays, ICC, IP, WB, ELISA, FC, IHC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody is specific for murine CCRL2 (formerly known as L-CCR), which is expressed on mast cells, activated macrophages, and endothelial cells. This receptor binds the leukocyte chemoattractant chemerin without triggering classical GPCR signaling, providing a specific mechanism for local enrichment of chemerin at inflammatory sites. CCRL2 presents active chemerin to leukocytes expressing the chemoattractant receptor CMKLR1/Chemr23. Chemerin can block anti-CCRL2 antibody binding. |
Alternative Name | CCR11; C-C chemokine receptor-like 2;Chemokine receptor CCR11; CKRX; CRAM-A; CRAM-B; G-protein coupled beta chemokine receptor; L-CCR; Lipopolysaccharide-inducible C-C chemokine receptor |
UniProt | O35457 |
Immunogen | This antibody was raised by immunising Wistar Furth rats with the mCCRL2 peptide/KLH conjugate. The immunizing amino-terminal mCCRL2 peptide with the sequence NH2- MDNYTVAPDDEYDVLILDDYLDNSC-COOH, corresponding to residues 1–24 of mCCRL2, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH). |
Application Notes | This antibody has been used in various FACS analyses, for instance, to confirm that it is only specific to mCCRL2-hemagglutinin (HA)/L1.2 transfectants and that peritoneal macrophages treated with LPS, TNFα, IFN-γ, or poly |
Antibody First Published | Zabel et al. Mast cell-expressed orphan receptor CCRL2 binds chemerin and is required for optimal induction of IgE-mediated passive cutaneous anaphylaxis. J Exp Med. 2008 Sep 29;205(10):2207-20. PMID:18794339 |
Note on publication | Describe the original generation of the anti-mCCRL2 mAbs BZ5B8 and BZ2E3 and verification of their binding specificity for murine CCRL2 by an ELISA-based assay. The antibodies were then used as part of the study which suggested that mast cell–expressed orphan receptor CCRL2 bound chemerin and was required for optimal induction of IgE-mediated passive cutaneous anaphylaxis. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
