Key features and details | |
Cat. No. | MABL-3505 |
Name | Anti-desmoglein-3 mAbs |
Clone No. | PVB16 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody specifically recognizes a conformational epitope in the EC5 domain of human desmoglein-3 (DSG This antibody was isolated as a human IgG1 from a patient with mucocutaneous pemphigus vulgaris and uses the VH3-23*01, D3-3*01, JH6*02, VL3-15*01and JL4*01. This antibody does not have in vitro and in vivo pathogenic activity, as assessed in a keratinocyte dissociation assay. |
Alternative Name | Pemphigus Vulgaris Ag; 130 kDa pemphigus vulgaris antigen; PVA; Cadherin family member 6; DSG3; CDHF6 |
UniProt | P32926 |
Immunogen | Made by immortalizing IgG-expressing B cells from Pemphigus vulgaris (PV) patients. |
Application Notes | |
Antibody First Published | Di Zenzo,G. et al. Pemphigus autoantibodies generated through somatic mutations target the desmoglein-3 cis-interface. J Clin Invest 122, 3781–3790 (2012). PMID:22996451 |
Note on publication | Describes the isolation and analysis of a panel of human antibodies from patients - inc data on ELISA, IF and in vitro and in vivo pathogenic activity assays. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
