Anti-DNA/RNA G-quadruplex mabs [BG4](MABL-3606)

Cat. No.

MABL-3606

Name

Anti-DNA/RNA G-quadruplex mabs  

Clone No.

BG4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

EMSA, ELISA, IF

Species Reactivity

Human

Basic Information

Specificity

Binds with high selectivity and low nanomolar affinity to DNA and RNA G-quadruplex structures.

Alternative Name

G4 quadruplex

UniProt

n/a

Immunogen

DNA/RNA G-quadruplex.

Application Notes

This antibody has been shown (by ELISA) to bind   selectively to G-quadruplex forming DNA and RNA structures. Please that   Fab-based versions as well as scFvs, with and without Flag-tag are available,   which needs to be considered when replicating published methods. Tsai et al.   (2022 et al. PMID: 36440760), report similar activtiy between whole-antibody   and scFv-based versions of BG4. Weldon et al. (2016, PMID: 27820800) used   Ab00174-1.1 to identify G-quadruplexes in long functional RNAs using an   electrophoretic mobility shift assay (EMSA). The mouse IgG1 version of BG4   was shown to have weak cross- reactivity with iMotif structures (2018, PMID:   29686376). iMotif structures can be detected selectively using our   anti-iMotif antibody Ab01462.

Antibody First   Published

Biffi G, Tannahill D, McCafferty J,   Balasubramanian S. Quantitative visualization of DNA G-quadruplex structures   in human cells. Nat Chem. 2013 Mar;5(3):182-6.PMID:23422559

Note on publication

Describes generation of a   G-quadruplex-specific antibody using phage display and the in vitro selection   (using ELISA and CD) of a structure-specific antibody probe that binds with   high selectivity and low nanomolar affinity to DNA G-quadruplex structures called BG4.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.