Anti-OX40/CD134 [OX86](MABL-3446)

Cat. No.

MABL-3446

Name

Anti-OX40/CD134 [OX86](MABL-3446)

Clone No.

OX86

From

Recombinant Antibody

Isotype

Engineer antibody

Application

agonist, ELISA, FC, IF

Species Reactivity

Mouse

Basic Information

Specificity

This antibody recognises murine OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes. It   is a member of TNFR-superfamily of receptors and acts as a costimulatory   molecule involved in long-term T-cell immunity.

Alternative Name

OX40; OX-40; Tumor necrosis factor receptor superfamily member 4; OX40 antigen; OX40L receptor; CD134; Tnfrsf4; OX-86

UniProt

P47741

Immunogen

This antibody was raised against the   recombinant mouse OX40 protein.

Application Notes

OX86 antibody, in its original rat IgG1 format, acts as an OX40 agonist in an Fc- dependent manner and has been   widely used in studies investigating the role of OX40 in tumor inhibition   (Hirschhorn-Cymerman et al., 2009). Studies comparing two recombinant murine Fc versions (IgG1 and IgG2a) elucidated the importance of the FcR engagement   in the antitumor activity of this antibody; the study demonstrated that a   stronger murine Fc (in an IgG2a version) was more effective in inducing a CT26 syngeneic tumor regression as well as it led to a stronger Treg depletion in the tumor in comparison with a murine IgG1 version (Metzger et   al., 2016). In another study two versions of the OX86 antibody (with a fully   functional Fc fragment and with an impaired Fc) and showed that Fc-dependent   activity was necessary in inducing systemic antitumor activity (Sagiv-Barfi   et al., 2018). FC: This antibody was also used in flow cytometry to stain   mouse CD4 and CD8 single-positive cells (al-Shamkhani et al., 1996). FC:   Blanquiceth et al. (2016) used this antibody in flow cytometry as well to   analyse cells coming from mediastinal lymph nodes and lung tissue. 

Antibody First   Published

al-Shamkhani A, Birkeland ML, Puklavec M, Brown   MH, James W, Barclay AN. OX40 is differentially expressed on activated rat   and mouse T cells and is the sole receptor for the OX40 ligand. Eur J   Immunol. 1996 Aug;26(8):1695-9. PMID:8765008

Note on publication

Describes the generation of the antibody, its use in flow cytometry (staining mouse CD4 and CD8 single-positive cells).

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.