Anti-GITR [DTA-1](MABL-3449)

Cat. No.

MABL-3449

Name

Anti-GITR [DTA-1](MABL-3449)

Clone No.

DTA-1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

agonist, IP, WB, FC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for murine GITR, a member of the tumor necrosis factor receptor (TNFR) family that is expressed at low levels on unstimulated T cells, B cells, and macrophages. Upon   activation, CD4+ and CD8+ T cells up-regulate GITR expression, whereas immunoregulatory T cells constitutively express high levels of GITR.

Alternative Name

CD357; AITR; GITR-D; Glucocorticoid-induced TNFR-related protein; TNFRSF18; Tumor necrosis factor receptor superfamily member 18

UniProt

O35714

Immunogen

This antibody was raised by immunising Wistar rats with the CD25+CD4+ T cell line. Hybridoma cells were generated by fusing spleen cells from the immunized rat with P3X63Ag8.653 myeloma cells, assessed   for secreting antibodies capable of neutralizing in vitro CD25+CD4+ T cell-   mediated suppression of anti-CD3-stimulated T cell proliferation9, and then   cloned. This DTA-1 clone secreted a monoclonal rat immunoglobulin G2a (IgG2a)   that showed high neutralizing activity.

Application Notes

This antibody has been used in various FACS   analyses for diverse immuno-oncological applications, such as to delineate  how membrane-organizing protein moesin controls Treg differentiation and   antitumor immunity via TGF-β signaling (Ansa-Addo et al, 2017), to suggest   the therapeutic potential of TGF-β-induced Tregs (iTregs) in treating   autoimmune gastritis (Nguyen et al, 2014), and to demonstrate that   costimulation with the ligand of GITR elicites dose-dependent enrichment for   cells of lower TCR affinity in the Treg cell repertoire (Mahmud et al, 2014).   This antibody has also been used in Western Blots to elucidate the mechanism   of human T-cell leukemia virus type 1 (HTLV-1) in the development of   neoplastic and inflammatory diseases (Satou et al, 2011), and in  immunoprecipitation analysis to aid the investigation of the role of GITR in   dominant immunological self-tolerance maintained by CD25+CD4+ regulatory T cells (Shimizu et al, 2002). In addition, the anti-tumour effects of this agonistic anti-GITR antibody have been demonstrated in various murine models   (Scirka et al, 2017; Coe et al, 2010; Cohen et al. 2010; Hu et al, 2008; Ko   et al, 2005).

Antibody First   Published

Shimizu Jun et al. Stimulation of CD25+CD4+ regulatory T cells through GITR breaks immunological self-tolerance. Nat  Immunol. 2002 Feb;3(2):135-42. PMID:11812990

Note on publication

Describe the original generation of this   antibody and its subsequent applications in FACS, immunoprecipitation, in   vitro and in vivo functional assays to show that GITR plays a key role in dominant immunological self-tolerance maintained by CD25+CD4+ regulatory T cells.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.