Anti-CD20 [18B12](MABL-3465)

Cat. No.

MABL-3465

Name

Anti-CD20 [18B12](MABL-3465)

Clone No.

18B12

From

Recombinant Antibody

Isotype

Engineer antibody

Application

depleting, functional assays, WB, FC, IF

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for murine CD20, which is a cell surface 33-37 (depending on the degree of phosphorylation)   kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells. Low number of the CD20   has been also detected on a subpopulation of T lymphocytes and it can be   expressed on follicular dendritic cells. Its expression on B cells is   synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated   calcium channel), cell cycle progression and B-cell proliferation. 

Alternative Name

Ly-44; B-cell differentiation antigen Ly-44;   B-lymphocyte antigen CD20; CD20; CD20 antigen; Lymphocyte antigen 44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning   4-domains, subfamily A, member 2

UniProt

P19437

Immunogen

This antibody was generated by immunizing CD20−/− mice with CD20-transfected cell lines (300.18 and 70Z3) and   CD20-peptide-keyhole limpet hemocyanin conjugate.

Application Notes

In the original publication, this antibody was used in FACS and in vivo functional assays to examine how B cell   depletion affects autoimmunity (Ahuja et al., 2007). In other studies, this   antibody has been used mainly in FACS, immunofluorescence, and various in   vitro and in vivo functional experiments. More recent works employing this   antibody have shown, for instance, that enhanced phagocytosis of circulating   B cells by Kupffer cells represents an important in vivo mechanism for improved activity of glycoengineered anti-CD20 mAbs (Grandjean et al., 2016),   that B lymphocytes limit senescence‐driven fibrosis resolution and favor   hepatocarcinogenesis in mouse liver injury (Faggioli et al., 2017), and that   B cells inhibit the antitumor immunity against an established murine   fibrosarcoma (Maglioco et al., 2017).

Antibody First   Published

Ahuja et al. Depletion of B cells in murine lupus: efficacy and resistance. JImmunol. 2007 Sep 1;179(5):3351-61 PMID:17709552

Note on publication

Describe the original generation of this antibody and its in vivo application to better understand how B cell depletion affects autoimmunity.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.