Key features and details | |
Cat. No. | MABL-3471 |
Name | Anti-PD-L2 [TY25](MABL-3471) |
Clone No. | TY25 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, Block, FC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody binds to mouse PD-L2 and competes with PD-1 binding. It does not cross-react with PD-L1. |
Alternative Name | CD273; B7-DC; PDL2; B7DC; programmed death ligand 2; CD273; Pdcd1lg2 |
UniProt | Q9WUL5 |
Immunogen | This antibody was raised by immunising SD rats with B7-DC-transfected RAW264.7 cells and selected on B7-DC-transfected NRK. |
Application Notes | This antibody has been used to detect murine PD-L2 by flow cytometry on stimulated dendritic cells as well as various mouse tumour cells. It also detects immunoprecipitates mouse PD-L2 and can be used to detect it by non-reducing Western Blot. TY25 inhibits PD-L2 binding to PD-1 transfected cells (Ansari et al. 2003, PMID: 12847137) and thereby blocks PD-L2: PD-1 signalling. |
Antibody First Published | Yamazaki et al. Expression of programmed death 1 ligands by murine T cells and APC. J Immunol. 2002 Nov 15;169(10): 5538-45 PMID:12421930 |
Note on publication | Describes the generation and initial characterisation of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
