Anti-CD226 [10E5](MABL-3476)

Cat. No.

MABL-3476

Name

Anti-CD226 [10E5](MABL-3476)

Clone No.

10E 5

From

Recombinant Antibody

Isotype

Engineer antibody

Application

FC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for murine CD226.

Alternative Name

DNAM-1; PTA1; PTA-1; platelet and T cell activation antigen 1; TLISA1

UniProt

Q8K4F0

Immunogen

This antibody was raised by immunising Lewis and Lou/M rats with the murine Th1 cell clone AE7 and Th1 cell lines derived from 5B6 and DO11.10 TCR transgenic mice, before screening for reactivity with Th1   clones and a lack of reactivity to Th2/Th0 cell lines.

Application Notes

The specificity of this antibody for murine   CD226 has been confirmed in flow cytometric analysis using 293 cells   transfected with CD226 cDNA and untransfected 293 cells (Dardalhon et al,   2005). This antibody has been used in flow cytometry to identify the surface   expression pattern of CD226, which is predominantly confined to T cells   (Dardalhon et al, 2005; Yasuma et al, 2016), and to detect NK cell CD226 expression and apoptosis (Huang et al, 2011). Immunisation of mice with this antibody inhibits antigen-specific T cell expansion (Dardalhon et al, 2005).   Additionally, treatment of experimental autoimmune encephalomyelitis (EAE)   mice with this antibody delays the onset and reduces the severity of Th1  cell-mediated autoimmune disease (Dardalhon et al, 2005).

Antibody First   Published

Dardalhon et al. CD226 is specifically   expressed on the surface of Th1 cells and regulates their expansion and   effector functions. J Immunol. 2005 Aug 1;175(3):1558-65. PMID:16034094

Note on publication

Describes the original generation of this antibody, and its characterisation.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.