Anti-CD27 [RM27-3E5](MABL-3480)

Cat. No.

MABL-3480

Name

Anti-CD27 [RM27-3E5](MABL-3480)

Clone No.

RM27-3E5

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, Neutralisation, FC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for the extracellular domain of murine CD27, a TNF-receptor superfamily member.

Alternative Name

TNFRS7; CD27 antigen; CD27L receptor; T-cell activation antigen CD27; Tumor necrosis factor receptor superfamily member 7

UniProt

P41272

Immunogen

This antibody was raised by immunising mouse   with mouse CD27-human IgG1 Fc fusion protein. Isolated popliteal lymph node   cells were then fused with P3U1 myeloma cells to produce stable hybridomas.

Application Notes

This antibody recognises murine CD27, as   confirmed by flow-cytometry and immunoprecipitation analysis (Sakanishi &  Yagita, 2010), a member of the TNF-receptor superfamily. The interaction   between CD27 and its ligand CD70, which is recognised by anti-CD70 antibody   TAN 1-7 (Ab00816), is crucial to regulation of the survival and   differentiation of T cells, B cells and NK cells. As a result, RM27-3E5   modulates multiple immune cell types. The original rat IgG2a version of RM27-3E5 did not deplete T cells, NKT or NK cells, but does enhance the   proliferation of anti-CD3-stimulated splenic T cells (Sakanishi & Yagita,   2010). In murine models of melanoma, it promotes the persistence of   tumour-specific CD8+ T cells within tumours, and reduces levels of PD-1 expression on CD8+ T cells (Roberts et al, 2010). Additionally, anti-CD27 reduces the   proportion of FoxP3+ CD4+ T cells in tumours, and enhances the effector activity of IFNγ-secreting, tumour-infiltrating CD8+ T cells and NK cells (Roberts et al, 2010). 

Antibody First   Published

Sumi et al CD27 and CD70 do not play a critical role in the development of experimental allergic conjunctivitis in mice Immunology Letters, Volume 119, Issues 1–2, 15 August 2008, Pages 91-96   PMID: 18579220

Note on publication

Describes the original generation of this   antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.