Anti-PD-1H [mam82](MABL-3486)

Cat. No.

MABL-3486

Name

Anti-PD-1H [mam82](MABL-3486)

Clone No.

mam82

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Block, ELISA, IHC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody specifically binds PD-1H.

Alternative Name

VISTA; VISR; B7-H5; B7H5; GI24; PP2135; SISP1; DD1alpha; C10orf54; chromosome 10 open reading frame 54; V-set  immunoregulatory receptor

UniProt

Q9D659

Immunogen

The antibody was generated by immunising a PD-1H-KO mouse with mouse PD-1H Ig fusion protein and adjuvant.

Application Notes

This antibody has been characterised by ELISA and by staining of PD-1H-transfected P815 mastocytoma. No background staining of WT or PD-1H-KO T cells was observed. This antibody inhibits WT   OT-II T cell proliferation at high OVA peptide concentrations (Flies D et al,   2014).

Antibody First   Published

Flies D et al. Coinhibitory receptor PD-1H preferentially suppresses CD4+ T cell–mediated immunity J Clin Invest. 2014  May 1; 124(5): 1966–1975. PMID:24743150

Note on publication

Describes the generation of the antibody and   its use to validate a coinhibitory function of PD-1H.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.