Key features and details | |
Cat. No. | MABL-3486 |
Name | Anti-PD-1H [mam82](MABL-3486) |
Clone No. | mam82 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Block, ELISA, IHC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody specifically binds PD-1H. |
Alternative Name | VISTA; VISR; B7-H5; B7H5; GI24; PP2135; SISP1; DD1alpha; C10orf54; chromosome 10 open reading frame 54; V-set immunoregulatory receptor |
UniProt | Q9D659 |
Immunogen | The antibody was generated by immunising a PD-1H-KO mouse with mouse PD-1H Ig fusion protein and adjuvant. |
Application Notes | This antibody has been characterised by ELISA and by staining of PD-1H-transfected P815 mastocytoma. No background staining of WT or PD-1H-KO T cells was observed. This antibody inhibits WT OT-II T cell proliferation at high OVA peptide concentrations (Flies D et al, 2014). |
Antibody First Published | Flies D et al. Coinhibitory receptor PD-1H preferentially suppresses CD4+ T cell–mediated immunity J Clin Invest. 2014 May 1; 124(5): 1966–1975. PMID:24743150 |
Note on publication | Describes the generation of the antibody and its use to validate a coinhibitory function of PD-1H. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
