Anti-CD25 [2E4](MABL-3492)

Cat. No.

MABL-3492

Name

Anti-CD25 [2E4](MABL-3492)

Clone No.

2E 4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Depletion, FC

Species Reactivity

Mouse

Basic Information

Specificity

2E4 recognizes mouse CD25. This epitope is distinct from the IL-2 binding site and does not block binding of IL-2 to   CD25. This antibody binds the same or overlapping epitope as 7D4 (Ab02216) but different than Ab01107. Murine CD25 is a 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high   affinity IL-2 receptor.

Alternative Name

IL-2-RA; Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2R subunit alpha; IL2-RA; p55

UniProt

P01590

Immunogen

The original version of this antibody was   raised to recognize mouse CD25.

Application Notes

2E4 is a non-IL-2 blocking anti-CD25 antibody. The variable fragment (Fv) of this antibody conjugated to a 38-kDa   portion of Pseudomonas exotoxin A exhibited exceptional ability to induce   tumor rejection and lasting antitumor immunitiy in mice making it a promising   canditate in the immunotherapy research and developemnt (Onda et al., 2019;   pmid: 30760587). This 2E4 Fv-based CD25- specific recombinant immunotoxin   (RIT) was injected into three different mouse tumor models and brought about   complete regressions of the majority of them leadinig, in many cases, to   immunity against tumors' reinjections and cross-tumor type immunity (Onda et   al., 2019; pmid: 30760587). Finally, it was demonstrated that this   immunotoxin (RIT) caused significant depletion of regulatory T cells (Tregs)   in the tumor environment but, importantly, not in the spleen; at the same   time, the number of effector, cytotoxic CD8 cells in the tumor increased what   would suggest the possible antitumour mechanism of this immunotoxin (Onda et   al., 2019; pmid: 30760587). 

Antibody First   Published

Ortega et al. The murine IL 2 receptor. I. Monoclonal antibodies that define distinct functional epitopes on activated T   cells and react with activated B cells J Immunol. 1984 Oct;133(4):1970-5.   PMID:6206144

Note on publication

Describes the characterization of the   antibody 2E4.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.