Cat. No.
MABL-3441
Application
inhibit, Block
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
2E6
From
Recombinant Antibody
Specificity
This antibody binds human notch 1.
Alternative Names
Notch1; hN1; TAN-1; Neurogenic locus notch homolog protein 1; Translocation-associated notch protein TAN-1
UniProt
P46531
Immunogen
The original antibody was generated by immunizing BALB/c mice with a concatemeric protein containing 4 repeats of amino acids 413-488 of human Notch 1 linked by two non-Notch 1 amino acids (AG linker).
Application Notes
The original human IgG1 version of this antagonist antibody binds notch 1 with an affinity of Kd= 8 nM. This antibody is capable of efficiently blocking the binding of all 4 ligands (Jag1, Jag2, DLL1 and DLL4) to notch 1. This antibody was also capable of specifically inhibiting Notch 1-dependent transcription. In vivo, this antibody was found to show little to no intestinal toxicity in mice. Hu2E6-62 caused decrease in functional angiogenesis by increasing vascular branching and decreasing vascular function. It inhibited angiogenesis induced by the human lung cancer Calu-6 cells. This antibody can be used to inhibit or reduced the proliferation and growth of tumors (WO2012003472).
Antibody First Published
WO2012003472 PMID:
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
