Anti-MBP mAbs [MBP-7128](MABL-3438)

Cat. No.

MABL-3437

Name

Anti-MBP mAbs [MBP-7128](MABL-3438)

Clone No.

MBP-7128

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA

Species Reactivity

Escherichia coli

Basic Information

Specificity

This antibody recognizes and binds maltose binding protein of E. coli.

Alternative Name

malE; MMBP; Maltose-binding periplasmic protein; Maltodextrin-binding protein

UniProt

P0AEX9

Immunogen

The original antibody was generated as a diabody by fusing two different Fv domains by short peptide linkers.

Application Notes

Maltose-binding protein   (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which   is responsible for the uptake and efficient catabolism of maltodextrins.   Maltose- binding protein (MBP) is one of the most popular fusion partners   being used for producing recombinant proteins in bacterial cells. MBP allows   one to use a simple capture affinity step on amylose-agarose columns,   resulting in a protein that is often 70-90% pure. In addition to   protein-isolation applications, MBP provides a high degree of translation and   facilitates the proper folding and solubility of the target protein (PMID: 20978971).

Antibody First   Published

Kim et al. Crystal structures of mono- and bi-specific diabodies and reduction of their   structural flexibility by introduction of disulfide bridges at the Fv   interface. Sci Rep. (2016); 6; 34515. PMID:27682821

Note on publication

Describes the crystal structure of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.