Key features and details | |
Cat. No. | MABL-3438 |
Name | Anti-IL-5 mAbs [JES1-39D10](MABL-3439) |
Clone No. | JES1-39D10 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | immunocytochemistry, Block, ELISA, FC, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognizes and binds human interleukin-5. |
Alternative Name | IL5; Interleukin-5; B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRF |
UniProt | P05113 |
Immunogen | The original antibody was generated by immunizing a male lewis rat with semi-purified recombinant mammalian-expressed IL-5. |
Application Notes | This antibody can effectively block IL-5 induced HL-60 histamine content and TF-1 proliferation. 39D10 was also evaluated for its ability to neutralize the ILJ-dependent growth of eosinophilbasophil colonies (i.e., both histamine-positive and histaminenegative Eo-type) in methylcellulose culture. This antibody did not cross-react with mouse IL-5 in indirect ELISA (PMID: 1706953). This antibody was used in immunohistochemical method to study the localisation and phenotype of individual cytokine producing cells in synovial biopsy specimens in rheumatoid arthritis (PMID:7677442). This antibody was also used in immunocytochemical and a flow cytofluorimetric method to detect intracellular IFN-g, IL-4 and IL-5 in T-cell clones, peripheral blood mononuclear cells PBMC and bronchoalveolar lavage fluid BALF cells (PMID: 9134033). The original rat IgG2a antibody binds human IL-5 with a binding affinity of Kd= 53 pM (PMID: 7613159). |
Antibody First Published | Denburg et al. Interleukin-5 is a human basophilopoietin: induction of histamine content and basophilic differentiation of HL-60 cells and of peripheral blood basophil-eosinophil progenitors. Blood (1991); 77(7):1462-8. PMID:1706953 |
Note on publication | Describes the use of this antibody to study the effect of recombinant human interleukin-5 on myeloid colony formation in methylcellulose and on alkaline passaged HL-60 basophilic cell differentiation. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
